This article has been cited by other articles in PMC. Abstract Aim Diabetes mellitus DM , a leading non communicable disease with multiple etiologies is considered as third greatest cause of death in all over the world. During DM, persistent hyperglycemia causes an increased production of free radicals via auto oxidation of glucose and non-enzymatic protein glycation which may lead to disruption of cellular functions and oxidative damage to membranes. The present study was designed to investigate the therapeutic effect of Evolvulus alsinoides on antioxidant activity in pancreas of experimental diabetes. Methods The antioxidant activities were done by using standard protocols.
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This article has been cited by other articles in PMC. Abstract Aim Diabetes mellitus DM , a leading non communicable disease with multiple etiologies is considered as third greatest cause of death in all over the world. During DM, persistent hyperglycemia causes an increased production of free radicals via auto oxidation of glucose and non-enzymatic protein glycation which may lead to disruption of cellular functions and oxidative damage to membranes.
The present study was designed to investigate the therapeutic effect of Evolvulus alsinoides on antioxidant activity in pancreas of experimental diabetes. Methods The antioxidant activities were done by using standard protocols. Results Oral administration of plant extract for 45 days resulted in significant antioxidant activity, increases the insulin level and also inhibits lipid peroxidation in pancreas of streptozotocin induced diabetic rats. The histopathological studies showed the normal histology of pancreas after treatment with plant extract and glibenclamide.
This study showed that the administration of Evolvulus alsinoides to streptozotocin induced diabetic rats improves the antioxidant activity and remodel the structure of pancreas due to the presence of secondary metabolites like phenols, flavonoids, alkaloids, steroids, terpenoids and glycosides in the ethanolic extract of plant material. Conclusion From the present study, it can be concluded that the plant extract effectively reduced the oxidative stress induced by streptozotocin and potentially increased the insulin level.
Hence, it can be used in the management of diabetes mellitus. Keywords: Streptozotocin, Evolvulus alsinoides, Pancreas, Antioxidant activity, Histopathological analysis Introduction In diabetes mellitus, chronic hyperglycaemia produces multiple biochemical sequelae and diabetes-induced oxidative stress that play an important role in the symptoms and progression of the disease [ 1 ].
Free radicals have been concerned in the causation of several disorders which includes diabetes and several other disorders [ 2 , 3 ]. Increased oxidative stress has been postulated in the diabetic state which coexists with a reduction in the antioxidant status [ 4 ]. Tissue antioxidant status has altered in diabetes resulting in increased oxidative damage of membranes and tissue injury [ 5 - 7 ]. Antioxidants may have great potential in ameliorating these disease processes and play an important role in protecting the human body against oxidative damage by free radicals [ 8 ].
Supplementation of therapeutics with antioxidants may have a chemo protective role in the diabetes [ 9 ]. Present therapeutic strategies mostly try to relieve the clinical manifestation of diabetes and complications. Since diabetes seems to be a stress - related disorder and the antioxidants may be useful in preventing the disorder.
Many plant extracts and its products have been shown to have significant antioxidant effect in treating these kinds of diseases [ 10 - 13 ]. Activity of important antioxidant enzymes like glutathione peroxidase and glutathione reductase, that directly scavenge free radicals or prevent their conversion to toxic products [ 14 ], is also altered in diabetic condition [ 5 ].
The control of different metabolic derangements is needed to prevent the development of diabetes and its complications [ 15 , 16 ]. Report of ethanobotany suggested that about medicinal plants possess antidiabetic potential and the bioactive compounds such as glycosides, alkaloids, terpenoids and flavonoids phenols are effective drugs both in preclinical and clinical studies [ 17 , 18 ].
Evolvulus alsinoides is a perennial herb belonging to the family of Convolvulaceae with a small woody and branched root stock. The plant is widely distributed in tropical and subtropical regions throughout the world. It grows commonly as a weed in open and grassy places throughout India, ascending to 6, fit. The herb was used to treat dysentery and to treat fever.
Mohammedan physicians used the plant as a general tonic to strengthen the brain and memory. It was used to treat bowel problems and to promote conception. The phytochemical screening of Evolvulus alsinoides also showed the presence of glycosides, alkaloids, terpenoids, steroids, phenols and flavonoids in the ethanolic extract. Hence, the present study was aimed to evaluate the effect of ethanolic extract of Evolvulus alsinoides on insulin level, antioxidant status and lipid peroxidation in the pancreas of streptozotocin induced diabetic rats.
Materials and methods Plant material The whole plant of Evolvulus alsinoides L. The plant was authenticated by Dr. Fresh plant material was washed under running tap water, air dried and powdered.
Sample extraction g of dried plant powder was extracted in ml of ethanol in an orbital shaker for 72 hrs. Repeated extraction was done with the same solvent till clear colorless solvent is obtained. The animals were under standard conditions and fed with rodent diet and water. Diabetes was identified in rats by moderate polydypsia and marked polyuria. Experimental protocol The animals were divided into five groups of six animals each.
Group I served as control; group II consisted of streptozotocin-induced diabetic rats; group III consisted of streptozotocin-induced diabetic rats treated with standard drug glibenclamide 1. Biochemical studies After 45 days of treatment the animals were sacrificed under chloroform anesthesia.
The blood was collected; plasma sample was used for insulin and C-peptide estimation by using kit method. Pancreas was quickly excised off, a portion of pancreas washed with saline and the homogenate was prepared using 0. The homogenate was centrifuged and the supernatant was used for the determination of lipid peroxidation [ 23 ], enzymatic antioxidant like superoxide dismutase [ 24 ], catalase [ 25 ], glutathione peroxidase [ 26 ], glutathione reductase [ 27 ], glucose 6 phosphate dehydrogenase [ 28 ] and non-enzymatic antioxidants like total reduced glutathione [ 29 ], vitamin C [ 30 ] and vitamin E [ 31 ].
The statistical analysis was carried out by one way analysis of variance using SPSS version 10 statistical analysis program. DM affects various organs in the body and it is responsible for many complications like diabetic neuropathy, nephropathy and retinopathy.
Hence the present study was undertaken to find out the therapeutic effect of Evolvulus alsinoides on antioxidants level in pancreas of streptozotocin induced diabetic rats. After treatment with whole plant ethanolic extract of Evolvulus alsinoides and glibenclamide for 45 days the values were reached near normal level as to that of control rats.
Table 1 Effect of ethanolic extract of Evolvulus alsinoides on insulin and C-peptide in plasma of control and experimental groups Groups.
The present investigation was undertaken to evaluate the histamine-induced bronchospasm of alcoholic extract of Evolvulus aslsinoides leaves in experimental animals. Bronchodilator activity of aqueous extract of Evolvulus aslsinoides leaves studied on the histamine aerosol induced bronchospasm in wister rats. Evolvulus aslsinoides leaves shows These results suggest that aqueous extract of Evolvulus aslsinoides leaves has not only bronchodilating activity but also decreases bronchial hyperreactivity by decreasing the infiltration of inflammatory cells in the airway and inhibition of release of histamine like mediators from the mast cell by stabilizing it.
Neuroleptic: reduce nervous tension by depressing nerve functions. Immunomodulatory: modifies the immune response or the functioning of the immune system. Nootropic: enhance memory or other cognitive functions. Nervine: calm the nerves. Psychostimulant: antidepressant or mood-elevating action. Sedative: calming or sleep-inducing effect.
Vishnukranthi (Evolvulus alsinoides) Information, Benefits and Uses